EFFECTS OF ELECTROMAGNETIC FIELDS EXPOSURE ON THE MAGNETOSOME PRODUCTION, ELIMINATION OF FREE RADICALS AND ANTIOXIDANT DEFENSE SYSTEMS IN MAGNETOSPIRILLUM GRYPHISWALDENSE MSR-1

Background and Aim:Magnetotactic bacteria integrate magnetosomes, which are unique organelles that contain nanometer-sized crystals of biogenic magnetic iron minerals with the ability to a response to the external magnetic fields. The biogenic magnetic nanoparticles (magnetosomes) was featuring tunable magnetism and high biocompatibility have been attracting much interest in medical applications especially as scavengers to eliminate intracellular reactive oxygen species. However, the physiological significance and other possible functions of these magnetosomes has not been explored in detail.Methods:In this study, we have investigated the biological functions of magnetosomes with respect to their ability to scavenge reactive oxygen species (ROS) in Magnetospirillum gryphiswaldense MSR-1. To assess the changes in ROS levels under different magnetic field intensity conditions, cells were cultured under the microaerobic condition in medium containing the high and low intensity of magnetic field.Results:The result showed that the antioxidant enzyme activity for eliminating of free radicals was increased by 38% when a magnetic field treatment with intensity 500 mT was added to the magnetosome biomineralization process of magnetotactic bacteria during of 50 h. The purpose of this study was to highlight the impact of magnetosome formation and antioxidant defense systems in the suppression of oxidative stress and elimination of free radicals in the magnetotactic bacteria cells.Conclusion:This is the first study to demonstrate that the magnetic field with inducing the magnetosomes production play an important role in decreasing or eliminating ROS. This is the first study to demonstrate that the magnetic field assisted magnetosome formation and antioxidants defense systems in Magnetospirillum gryphiswaldense MSR-1.

Keywords:

elimination of ROS , antioxidant enzymes activity , magnetic field , magnetosome , Magnetospirillum gryphiswaldense MSR-1