DETECTION OF HELICOBACTER PYLORI IN THE PATIENTS WITH GASTRIC CANCER BASE ON VARIOUS VIRULENCE GENES USING MULTIPLEX PCR

Background and Aim:Background: It is estimated that Helicobacter pylori colonizes the stomachs of half the world’s population and cagA-positive strains are present in 60–70% of infections in Western countries. Our aim was to determine the prevalence of the cagA/E/T, vacA and hrgA in H. pylori isolates among patients with gastric cancer (GC) in Karaj, Iran.Methods:: A total of 50 non-repeated gastric biopsies obtained from patients undergoing endoscopy in Shahid Fayazbakhsh endoscopy center. The presence of cagA/E/T, vacA and hrgA genes were determined by multiplex-PCR method.Results:Of 50 gastric biopsies, 44 (88%) samples were positive for various H. pylori virulence genes. Molecular analysis of these virulence factors showed that th: A total of 50 non-repeated gastric biopsies obtained from patients undergoing endoscopy in Shahid Fayazbakhsh endoscopy center. The presence of cagA/E/T, vacA and hrgA genes were determined by multiplex-PCR method. e frequency of cagA, cagT, cagE, vacA and hrgA were 16 (32Conclusion:: The presence of different pathogenic genes has considerable effects in causing gastric ulcer, peptic ulcer, and gastric cancer. The effects of other genes, such as hrgA, in tissue damage and inflammation response are markedly important.

Keywords:

pylori , cagA/E/T , vacA and hrgA