CELL EPITOPE-BASED VACCINE CANDIDATE AGAINST BACTEROIDES FRAGILIS BY IMMUNOINFORMATICS APPROACH
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
View: 423
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
MEDISM19_540
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:Bacteroides fragilis is one of the most abundant anaerobes found in humans and cause intra-abdominal infection, sepsis, soft tissue abscess and bacteraemia. The traditional approach to design a vaccine is a long process that involves the attenuation of the pathogen through sub-culturing followed by its administration. The administration of whole pathogen raises several safety issues and toxicity owing to the unwanted biomaterial. Thus, peptide can also serve as a vaccine candidate, as well as can be used for designing immunotherapeutic. It has been shown in the past that a small peptide or antigenic regions can also activate different arms of the immune system. The conventional approach for designing vaccine against a particular disease involves stimulation of the immune system using the whole pathogen responsible for disease.Methods:First, vaccine targets were selected according to standard characteristic. Then B-cell epitope of canditate peptide were predicted using well-known online bioinformatics server (IEDB, Bcepred, Ellipro, DiscoTope) and then were selected and compared based on the highest score and the highest repetition.Results:In this study, we designed a new structural model containing two putative antigenic determinants of SusC/RagA family TonB-linked outer membrane protein and membrane protein insertase YidC fused together by hydrophobic linkers.Conclusion:The fusion peptide construct may be used as epitope-based vaccine for development of Bacteroides fragilis vaccine candidates.
Keywords:
Authors
Shirin Dashtbin
Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran
Ramin Abiri
School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Amirhooshang Alvandi
School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran