USING THE MULTIPLEX-PCR METHOD FOR DETECTION FOUR VENEREAL BACTERIA (TAYLORELLA EQUIGENITALIS، KLEBSIELLA PNEUMONIAE، PSEUDOMONAS AERUGINOSA AND STREPTOCOCCUS ZOOEPIDEMICUS) FROM THE MARE’S CLITORAL SAMPLES

Background and Aim:Sexually transmitted diseases are one of the major problems in reproductive management of horses. These diseases cause large economic losses to horse and horse breeding. The aim of this study was to design a multiplex-PCR method in order to detect four bacterial sexually transmitted diseases (Tylerella echinogenitalis, Klebsiella numonia, pseudomonas aerogenosa and Streptococcus zoepidemicus).Methods:For this study, we sampled from the Clitoris of 106 horses using sterile swab. The primer design was based on the specific genes of each bacterium and was designed to be eligible for multiplex PCR. DNA extraction from each sample was performed using a special extraction kit. Pure sample from each bacterium were used as positive control and the PCR reaction conditions continued until favorable results were obtained in multiplex conditions on positive control samples.Results: Regarding the accuracy of the positive control and PCR conditions, all samples were free from the sexually transmitted factors and no bacteria were isolated.Conclusion: Multiplex-PCR responses to identify the four bacterial sexually transmitted factors can be designed and performed, although the samples taken in this study were free of bacteria.

Keywords:

Taylorella equigenitalis , Klebsiella pneumonia , pseudomonas aeruginosa , Streptococcus zooepidemicus , multiplex-PCR