ISOLATION AND IDENTIFICATION OF MYCOBACTERIUM CAPTURED FROM MICE FROM INFECTED FARM BY TUBERCULOSIS

Background and Aim:Bovine tuberculosis is one of the most important zoonotic diseases in Bovidae. The humans and animals that transit to the farm can transfer Mycobacterium bovis to cattle, so the aim of this study was evaluation the possible role of mice in transferring the Mycobacterium infection in dairy farms.Methods:From a dairy cattle farm with more than 10 percent infection of Mycobacterium bovis, five mice were captured and their liver, spleen, and lung cultured in the LJ medium. The Acid-Fast staining of the isolates prepared to identify Mycobacterium and PCRs were carried out afterwards.Results:Three Out of five cultures were positive in direct smear by Acid Fast staining and in PCR-16SrRNA, which indicate that mentioned isolates belonging to Mycobacterium genus. Also positive PCR-IS6110 confirmed that isolates are one of species as Mycobacterium tuberculosis Complex. Currently, we are conducting PCR-RFLP and RD Typing to exact identification of these isolates.Conclusion:Animals such as mice and cats that live in the farm can harbor Mycobacterium. In this study, it has been proven that mice certainly transfer Mycobacterium to cattle farms.

Keywords:

Mycobacterium tuberculosis Complex , Mycobacterium bovis , PCR IS6110 , 16SrRNA