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Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy People

تعداد صفحات: 1 | تعداد نمایش خلاصه: 121 | نظرات: 0
سال انتشار: 1395
کد COI Paper: IPMCMED01_078
زبان Paper: Englishglish
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مشخصات نویسندگان Paper Evalition Expression of mir-338-3p in Chronic Lymphocytic Leukemia Patients and Healthy People

Arman Akbarpour - Department of Basic Sciences, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
Mansoor Salehi - Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Parisa Mohammadinejad - Biology Dept., Shahr e Kord Branch, Islamic Azad University, Shahr e Kord, I.R. of Iran

چکیده Paper:

Background: microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. Chronic lymphocytic leukemia (CLL) is characterized by an accumulation of mature CD5+ B cells that are dependent on micro environmental support, and genetic mutations. The aim of the present study was to compare the expression level of mir-338-3p in normal and neoplastic samples from CLL patients by RT-qPCR.Methods: In the present case-control study, 15 blood samples from patients with CLL and 15 blood samples from healthy group under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings were collected. After extracting RNA from normal and tumor blood samples, cDNA synthesis method according to the protocol and RT-qPCR was performed. mir-338-3p expression level was calculated using ΔΔCT. All data were analyzed using SPSS software.Results: The results of RT-qPCR indicated that miR-338-3p down-regulation was significantly correlated with CLL cancer clinic pathological features. Expression level of mir-338-3p was 25.68% and 74.32% in CLL patients and healthy group respectively (p value=0.001).Conclusion: Due to the previous reports, mir-338-3p act as tumor suppressor in CLL, which could be used as biomarker for CLL diagnosis. In this study we propose that miR-338-3p is a potential diagnostic marker and therapeutic target of CLL.

کلیدواژه ها:

CLL, microRNA, mir-338-3p, RT-qPCR

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کد COI Paper: IPMCMED01_078

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برای بار اول: (1395, Akbarpour, Arman؛ Mansoor Salehi و Parisa Mohammadinejad)
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