Distribution of various pathogenic bacteria in Mehr hospital using BD BACTEC Blood Culture and Phoenix technology

Background: To determine the prevalence of bacterial pathogens in different clinical specimens from various wards of Mehr hospital, Mashhad, 2017-2018 Methods: This study was conducted in Mehr Hospital laboratory between September 2017 and March 2018. 10 wards of Mehr Hospital were selected to provide samples of different specimen types. In the laboratory, samples were injected in BACTEC vials and insert in the BACTEC Instrument. The instrument alarm indicated if the sample was positive. Positive samples were cultured in special medium for different specimen types. After 18 to 24 hours, bacterial colonies were identified using the BD Phoenix automated identification and susceptibility testing system. Results: From the original 214 isolate samples, 96 bacterial isolates were identified. The most common isolates were Escherichia coli (E. coli)--30%. Enterococcusspp. -19 %, and Staphylococcusaureus -15%. Others were Klebsiellapneumoniae.-14.5%, Acinetobacterspp.— 7.4%, Enterobactercloacea --6.5%. The least common were pseudomonas aeroginosa—3.3%, KlebsiellaOxytoca –2.3%, Morganellaspp --1% and Burkholderiaspp 1%. The most common clinical samples from which isolates were collected included urine (20.8%), Bronchia (20.8), fluid (15.6%), blood (12.5%), Wound (11.5%), tissue (6.2). Others were abscess (4.2%), catheter (3.1) and other specimen (5.3%) The most common wards from which isolates were collected were: surgery (39.5%), Intensive Care Unit (ICU) Open Heart (25%), ICU General (10.4%), Coronary Care Unit (CCU) (7.3%), Neonatal ICU (5.2%), Heart (4.2%), Post ICU (4.2%), and other wards (4.2%). Conclusion: This study indicates an increasing prevalence of bacterial pathogens in Mehr hospital. Escherichiacoli (E. coli), Enterococcus spp. and Staphylococcus aureus, which are the most life-threating bacteria for humans, were identified as the common pathogens.Surgery and Intensive Care Units (ICU) Open Heart had the most pathogens. Based on these results, it is extremely important to consider implementing control strategies and additional measures to prevent the spread of these bacterial pathogensin hospital environments, especially in the aforementioned wards.

Distribution of various pathogenic bacteria in Mehr hospital using BD BACTEC Blood Culture and Phoenix technology Keywords:

BACTEC , Phoenix technology , pathogenic bacteria