Comparison of PCR and culture methods to determine Mycoplasma hominis in woman s endocervical samples referred to infertility hospital of Hamadan

Background: Mycoplasma hominis bacterium of Mycoplasmataceae family and is commensal in the female genital tract. With surveys conducted in recent years, to the presence of these potential pathogenic bacteria in the human body and in disorders such as vaginitis, infertility, abortion, preterm delivery and other diseases have been mentioned. The aim of this epidemiological-descriptive analytical research, the frequency of Mycoplasma hominis in the patient s women referred to Hamadan Fatemyeh Hospital in 2016. Methods: In this research, from 234 women patients with have at least one of disorders symptoms such as vaginitis, infertility, abortion, preterm delivery, by using the swab, endocervical samples taken. By eliminating the filtration medium and PCR molecular techniques to trace the 16s rRNA gene used as bacterial diagnostic methods. Descriptive statistics were used to analyze the data. Results: The prevalence of Mycoplasma hominis in the study population was 13.7% in both methods. Relationship of infection with a number of disorders mentioned above was important so that 10.3% of patients with infertility and 12.3% of them colonized with vaginitis by Mycoplasma hominis. Agreement coefficient between culture and PCR methods for the detection of this bacteria is very high (k = 0.5). Conclusion: The prevalence of Mycoplasma hominis in the study population is significant, so further investigation is necessary. Although molecular techniques such as PCR to detect these bacteria are very sensitive, but still, culture method has sensitive desirable

Comparison of PCR and culture methods to determine Mycoplasma hominis in woman s endocervical samples referred to infertility hospital of Hamadan Keywords:

Mycoplasma hominis , infertility , vaginitis , abortion , Culture , PCR