Human Unrestricted Somatic Stem Cells (USSCs) Attenuate Systemic Inflammation in the Mouse Model of ALI

Background and Aim: Sepsis is the oldest life-threatening challengein medicine with difficult condition to diagnose and treat. The thirdInternational Consensus Sepsis is defined as unregulated host reactionto infection, characterized by systemic inflammation. Regarding thecomplicated pathophysiology, the management of sepsis is a veryconsiderable procedure in the hospital’s intensive care unit (ICU).Cell therapy is a promising option that shows significant outcomesin inflammatory diseases. In the present study, we investigated thetherapeutic potential of Human unrestricted somatic stem cells (USSCs)isolated from the umbilical cord in the mouse model of ALI.Methods: ALI model was established via injection of LPS. Mice wererandomized into three groups: (1) LPS group, (2) LPS+ PBS group, and (3)LPS+ USSC group. The mice were sacrificed at 24 hours after injection.Serum was collected for cytokine concentration measurements, lung andliver harvested for evaluation of injury, and histology.Results: USSCs improved survival rate and suppressed concentrations of pro-inflammatory mediator’s TNF-α, and IL-6 and the level of antiinflammatorycytokine IL-10. The cells showed significant improvementin lung and liver injury, edema and hepatic enzymes.Conclusion: USSCs significantly enhanced the survival rate of micesuffering from ALI and considerably reduced systemic inflammation.USSCs can be the appropriate therapeutic approach in the managementof sepsis throw the anti-inflammatory effects.

Keywords:

Acute lung injury , Umbilical cord , Unrestricted Somatic Stem Cells , Inflammation , Cell therapy