Screening and identification of halophilic and halotolerant bacteria producing extracellular lipase from West Mazandaran

Hydrolytic enzymes of halophilic bacteria such as lipase are of particular importance in the industry due to their high stability over industrious hard conditions, such as high temperatures, various pH changes, and salinity levels. In order to screen and purify the halophiles, a selective MH medium was prepared. By analyzing the optimum salt growth, 40% of the purified strains of moderate halophiles and 60% were halotolerant. The optimum growth temperature of all isolates was determined at 25-45°C. After analyzing the morphology and salinity of isolates, biochemical tests, as well as the ability to produce 7 hydrolysis enzymes, were investigated. Approximately 40% of the isolates were able to produce extracellular lipase enzymes, the major producers of which were gram-positive bacteria. The lipase enzyme activity of one of the isolates was measured by titration. In this method, olive oil was used as a substrate. The activity of the lipase enzyme measured by the strain was 1.37 U/ml. The results show that some isolates have the ability to produce several enzymes, and this will depend on the medium culture in which surrounding the bacterial colonies

Keywords:

Lipase , Extracellular Hydrolytic Enzymes , Halophilic bacteria , Halotolerant bacteria , West Mazandaran