Synthesis, characterization and antimicrobial activity of zinc oxide nanoparticles prepared by a green approach based on extract of Seidlitzia rosmarinus after microwave and maceration extraction method

Microwave-assisted extraction (MAE) has been accepted as a potential and powerful alternative for the extraction of organic compounds from plant materials. This extraction technique with shortened extraction time, reduced solvent consumption, increased pollution prevention, and with special care for the constituents has gained attention [1].Seidlitzia rosmarinus is a perennial woody plant grown mostly along the banks of salt marshes and in soils with high saline water tables [2]. In this research, the aqueous extract of Seidlitzia rosmarinus plant was obtained in the various frequencies of microwave irradiation (90 and 270W) as well as maceration methods. The obtained extracts were used as a natural stabilizer and capping reagent for green synthesis of zinc oxide nanoparticles. Characterization of as-prepared ZnO NPs was carried out by various techniques such as SEM, XRD, UV-Vis absorption and fluorescence spectroscopics (Fig.1). In addition, antimicrobial activities of the samples were investigated with well diffusion (WD) technique; minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. The antibacterial testing showed that the sample has acceptable antibacterial activity. Anti-bacterial properties of the samples prepared by MAE were more than maceration method.