Binding of small molecules to proteins: An old problem with new insights

In characterization, various aspects of macromolecular phenomena in living systems,reversible binding of small molecules to biological macromolecules such as proteins and DNA is of fundamental importance. This is illustrated by the binding curve that measured experimentally and analyzed by the binding models in order to characterize its physical nature and biological importance. This article represents the results of more than 20-year research and study on experimental, theoretical, and computational aspects of this phenomenon in our group. Accordingly, the novel analyzing models and experimental measuring techniques are introduced and the contribution of these works on improvement of our understanding of the functional chemistry of proteins is discussed.