Evaluation of Endometrial Thickness Changes of ovariectomized Rats Induced by GONAL -F with and without HCG

Objective: To attach the fetus to the endometrial epithelium, the endometrium should be affected by the steroid hormones secreted from the ovary and allow do this. Thus the endometrium is then subject to several structural and biochemical changes. Implantation involves the embedment, adhesion and invasion steps that specific receptors play a role in them. The role of membrane receptors in uterine epithelium is important. Most of these receptors are glycoprotein and among which integrins are of importance. In period, the uterus undergoes structural changes so that endometrium is provided for the placenta and the embryo in addition to extracellular matrix especially fibronectin. Material & method: For surgery the animals are anesthetized with ketamine and xylacin intraperitoneally. After opening the abdomen, the ovaries are removed from both sides. After 72 hours, and observing the vaginal plaque, animals received high doses of ketamine and xylazine. The left uterine horn is removed and placed in fixative solutions and during the tissue processing of the molds are prepared to be cut. The slices are stained with PAS to determine the thickness of the glycoprotein layer. Result: There is a statistically significant difference in the increase in the thickness of the luminal membrane of glycoprotein layer of endometrial epithelium cells between the GONAL -F receptor groups with HCG compared to other groups at the same time. Conclusion: The results of this study showed that GONAL -F and HCG can increase the amount of glycoprotein expression and not damage the endometrial wall.

Keywords:

Gonal-F , HCG , Glycoprotein , Endometrium , Receptor , Epithelium