Overexpression of High-Mobility Motor Box 1 in the Blood and Tissues of Patients with Head and Neck Squamous Cell Carcinoma
Publish place: Iranian Journal of Otorhinolaryngology، Vol: 30، Issue: 5
Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
View: 427
This Paper With 11 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJOTO-30-5_003
تاریخ نمایه سازی: 12 تیر 1398
Abstract:
Introduction:
Head and neck squamous cell carcinoma (HNSCC) is one of the most common cancers in the world. Extra- and intra-cellular high-mobility motor box 1 (HMGB1) proteins are invovled in the pathogenesis and prognosis of cancer. Regarding this, the present study was conducted with the aim of investigating the expression of HMGB1 protein and mRNA levels in the blood, tumor tissue, and marginal normal tissue of patients with head and neck squamous cell carcinoma (HNSCC)usingthequantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC).
Materials and Methods:
This study was performed on 88 patients with HNSCC, who referred to the otorhinolaryngology and oral pathology departments, affiliated to Mashhad University of Medical Sciences, Mashhad, Iran, and a group of healthy subjects (i.e., control group) matched in terms of age and gender.RNA was collected from fresh tumor tissues, marginal tissues, and blood, followed by the implementation of quantitative PCR on the specimens.Furthermore, the expression of HMGB1 in tumor and normal margins was evaluated by means of IHC. Thedata were analyzed in SPSS software.
Results:
According to the results,the expression levels of HMGB1 protein and mRNA were significantly higher in the tumor tissue than in the normal margin tissues (P<0.01).In addition, there was a significant correlation between histologic grading and the expression of HMGB1 protein and mRNA in tissues (P<0.05). Furthermore, the receiver operating characteristic curve of the HMGB1 mRNA in tissue was located closer to the theoretical 100% sensitivity.
Conclusion:
The findings revealed a higher increase in the levels of mRNA and HMGB1 protein in HNSCC, compared to those in the normal margin tissues. In addition, HMGB1 mRNA showed a significant expression in the tissue and blood of the patients with lymph node involvement.
Keywords:
Biomarker , Early diagnosis , Head and neck squamous cell carcinoma (HNSCC) , High-mobility motor box (HMGB1) , Lymph node involvement
Authors
Farnaz Mohajertehran
Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Hossein Ayatolahi
Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
kamran khazaeni
Department of Otorhinolaryngology, Mashhad University of Medical Sciences, Mashhad, Iran.
Mohammad -Taghi Shakeri
Department of Biostatic, Mashhad University of Medical Sciences, Mashhad, Iran.
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :