GC-MS Analysis of phytocomponents and antioxidant, antimicrobial activities of aerial parts of Stachys turcomanica

The aim of this study was to investigate the Stachys turcomanica phytochemical compounds, antimicrobial and antioxidant activity of methanolic extract. The volatile constituents from the aerial parts of Stachys turcomanica growing wild in Iran were obtained by hydrodistillation and analyzed by GC and GC-MS. In the GC-MS analysis, Forty-six components representing 81.1% of the oil were identified. The main constituents the oil were 1-octen-3-ol (13.4%), β -pinene (7.9%), α -pinene (5.6%), α -bisabolol(4.4%), ar-curcumene(4.0%) and β –myrcene(3.7%).The phytochemical analysis revealed the presence of alkaloids , flavonoids and terpenoid in varying concentration . The antioxidant activity of aerial parts of methanolic extract was studied in vitro by 2’2’-diphenylpicrylhydrazyl (DPPH) radical–scavenging activity. The methanolic extract of Stachys turcomanica leaves exhibited amaximum DPPH scavenging activity of (81.61±0.78) %at10mg/mL followed by aerial parts of plant. Whereas for BHT (standard) was found to be (94.79±0.75) % at the same concentration. Methanol solvent to screen the antimicrobial activity selected phytopathogens by agar diffusion method. The maximum antibacterial (phytopathogene) activities were observed on Xanthomonascampestrispv.Campestris,Agrobacterium sp.and Pseudomonas viridiflava.

Keywords:

Stachys turcomanica , phytochemical , hydrodistillation , methanolic extracts , antimicrobial and antioxidant activity