Evaluation of TGF β1, VEGF and Myofibroblast in Oral Mucosal and Skin Wound Healing : An Experimental In-Vivo Study

Introduction: In cutaneous injuries, scar formation is a part of skin repair process. Transforming growth factor beta 1 (TGFB1) induces myofibroblast differentiation that plays an important role in wound contraction. Vascular endothelial growth factor (VEGF) increases vascularization during wound healing. The purpose of this study was to compare the changes in levels of TGFB1 and VEGF in the skin and oral mucosal wounds in mouse model.Methods:In this experimental study,tissue samples were collected from the skin and oral mucosal tissue of 50 (BALB-c, aged 6-8 weeks) mice and placed in RMPI + 5% FBS culture medium. Culture fluid was obtained at 0-24 and 0-48 h intervals and evaluated with ELISA (R&D) kit to measure the concentration of TGF-B1 and VEGF. Also, the samples were stained with IHC for detection of myofibroblast marker SMA-α.Data were analyzed byKruskal-Wallis, Chi Square, Fisher and independent sample t tests. Results: The mean number of myofibroblasts in the skin was more than that in oral mucosa (P <0.001).TGFB1 and VEGF concentrations in the skin were more than those in oral mucosa (P < 0.001).Conclusion: Reduction in VEGF and TGFβ1 concentrations and number of myofibroblasts may beresponsible for less scar formation in the oral mucosa.

Keywords:

Wound healing , TGFB1 , VEGF , Myofibroblast