Evaluation of Sperm Abnormal Morphology in Male Rats Induced by Varicocele

Background: Varicocele refers to the abnormal inflation of spermatic veins in the scrotum. Varicocele is a common cause of infertility in men who are clinically associated with sperm abnormal morphology. So, this study aimed to evaluation sperm abnormal morphology in male rats induced with varicocele ex-perimentally.Materials and Methods: In this study, 30 adult male Wistar rats were divided into three groups, I: varicocele-induced II: sham and III: control. After two months of varicocele induction, rats were sacrificed and epididymides were dissected. Then, Sperm abnormal morphology assessed by WHO protocol and eosin-nigrosine test. Differences within groups were compared by one-way analyses of variance (ANOVA) using a post hoc test (Tukey). Collected data were presented as mean ± stand-ard error of mean (SEM) and P < 0.05 was considered to be significant.Results: The result of this study showed that mean Sperm ab-normal morphology were significantly higher in varicocele in-duction group compared to control and sham groups (P<0.01). The deformity of tail and head of sperm in varicocele rats com-pared with sham and control rats showed a significant increase of (P<0.05). However, that sperm neck deformity in varicocele groups increased but did not show significant difference.Conclusion: In this study, by evaluating sperm parameters be-tween groups, it was shown that the abnormal morphology of sperm in varicocele rats significantly increased, which was con-sistent with previous studies. Also, respectively highest sperm anomalies were the sperm tail and head, and then sperms that had been defeated from the neck was in the next rank.

Keywords:

Varicocele , Sperm Parameters , Sperm Abnormal Morphology , Eosin - Necrosin Test