Investigation of The Level of Insulin-Like Factor 3 in Fetal Amniotic Fluid in Second Trimester in Pregnant Women Referring to Treatment Clinic in Ardabil Province

Background: By increasing the age of marriage and the ges-tational age, the probability of infertility and the formation of embryos with genetic problems and the birth of newborns with congenital anomalies also increases. In such circumstances, the use of diagnostic methods for the health of the fetus dur-ing pregnancy is emphasized to prevent the risk of the birth of children with abnormalities of the nervous system, and in particular Down syndrome. One of these diagnostic methods is amniocentesis, which is done before birth, and usually between 15 to 25 weeks of gestation, to examine genetic data and to de-tect abnormalities in the birth and sex of the fetus. Different fac-tors can be titrated and diagnosed by amniocentesis, including amniocentesis and human insulin-like factor-3 levels, and tes-tosterone levels in amniotic fluid in male and female embryos and analysis. The relationship between this factor and testicular loss, The sex of the fetus, testosterone and other hormones.Materials and Methods: Of pregnant women who were can-didates for amniocentesis, 109 pregnant women entered writing with written consent. Sampling of amniotic fluid was carried out under the supervision of a prenatal practitioner and samples were transferred to the lab in refrigerated conditions to be ana-lyzed and evaluated.Results: Data analysis revealed that there is a significant rela-tionship between maternal weight and age, and the amount of testosterone in the fetus. As the age and weight of the mother in-crease testosterone levels, there is a direct relationship between testosterone and insulin-like factor- 3.Conclusion: Testosterone is one of the most important steroid hormones in the human body, and its production begins in the mother s womb before the second month of the embryo, which plays a role in determining the sex of the fetus, and the main source of its secretion in the male body is testicular. There is a relationship between this hormone and the insulin-like factor-3. The insulin-like factor plays an important role in the decline of the testicles in the fetus, so timely diagnosis and treatment of these disorders is important.

Keywords:

Amniocentesis , Insulin- Like Factor- 3 (INSL3) , Tes-ticular Depression , Testosterone (T)