The Effect of L-Carnitine on The Ovarian Histology in Mouse Model of The Endometriosis

Background: . Endometriosis is a common disease in women that is characterized by the presence and growth of endometrial tissue, having blood vessels in abnormal locations outside the uterine cavity, which is closely related to infertility and causes complications and abnormalities in the ovary. Improving endo-metriosis can help improve fertility. L-carnitine, based on the improvement of metabolism and antioxidant and anti-inflam-matory effects, can be a solution to improve the complications of endometriosis on the ovary and improve histology. The pur-pose of this study is to investigate the effect of L-carnitine on the ovarian histology in mouse model of endometriosis Materials and Methods: At the first stage, induction of endo-metriosis in mice was done by injection of suspension of endo-metrium separated from the uterus of donor mice. In the sec-ond stage, the control group was divided into four treatments: control without any treatment, control receiving L-carnitine intrapritonally (250mg/1kg, every day), control receiving da-nazol intrapritoneally (25mg/1kg, every other day) and control receiving concurrently L- carnitine and danazol. The endome-triosis group was divided into four treatments: endometriosis without any treatment, endometriosis receiving L- carnitine intrapritoneally (250mg/1kg, every day), endometriosis receiv-ing danazol intrapritoneally (25mg/1kg, every other day), and endometriosis receiving concurrently L-carnitine and danazol. At the end of the treatment period, the mice were dissected then ovaries separated and histologically examined.Results: Induction of endometriosis cusses problems in ovary include reducing the number of folliculogenesis, decreasing the number of different follicles and their quality, decreasing the quality and health of the ovum, increasing the number of cysts and bleeding in the ovary and adhesion of the ovary to the tis-sues and surrounding pelvic organs. Treatment with l-carnitine lead to reducing the number of ovarian cysts, increasing the number of types of follicles especially the antral follicles and graph, reducing ovarian hemorrhage. Also, treatment with dan-azol reduceing the number of ovarian cysts, increasing total fol-licles, and reducing the amount of bleeding. Co-administration of danazol and l-carnitine also improved the condition of the ovary, as well as increasing follicular growth and ovulation in the endometriosis ovary.Conclusion: Therefore, it can be concluded that danazol and l-carnitine can improve the condition of the ovary compared to the endometriosis group, which can improve the complications of the disease.

Keywords:

Ovary , Endometriosis , L-Carnitine , Mouse