Antibiotic Resistance in Escherichia coli Isolates from Patients with Urinary Tract Infections referred to Neka Laboratories-Iran

Background and Objectives:Urinary tract infection (UTI) is an inflammatory response of the urethra to the invasion of pathogenic microorganisms. This infection is of the highest importance after respiratory tract infection and isthe second most common infection in children and adults and the most important in infants. Matherials and Methods:This descriptive cross-sectional study was conducted from December 2016 until the end of September 2017 in the Neka medical diagnostic laboratories, located in Mazandaran province. To do so, 8530 urine samples were collected in sterile containers by Midstream method from patients referred to laboratories. Morphological study and identification of isolated bacteria by using gram staining and differential biochemical tests were performed. Antibiotic resistance of bacteria was determined by Disk diffusion method in Muller Hinton Agar medium. Then, the antibiotic disks of ciprofloxacin, sulfamethoxazole, nalidixic acid, norfloxacin, gentamicin, ceftriaxone, cefotaxime and imipenem provided from the padtanTeb Co. were placed at a distance of at least 2.5 cm from each other on the medium and incubated for 24hours 37 C. Results:Of the 720 positive samples, 620 (86/8) samples were for women and 95(13/19) for men. The main cause of E.coli was urinary tract infection which was isolated in 352(51/6) cases. Escherichia coli isolates showed the highest and lowest resistance, respectively, to sulfamethoxazole (49.72%) and Nalidixic acid (39.77%) and the highest sensitivity to Gentamicin (61.93%) and ciprofloxacin (48.86%). Conclusion:The results of this study indicate an increase in the resistance of the strains of E.coli to the sulfamethoxazole and nalidixic acid antibiotics, which may be due to the overdose of these antibiotics. The report of antibioticsusceptibility in this area can be considered by physicians in empirical treatments.