Flaviviruses screening in mosquitoes from Dorud County, Lorestan Province, Iran

Culexpipienscomplex are important to transmission of St. Louis encephalitis, West Nile, Sindbis, and Rift Valley fever viruses. Screening of mosquitoes for viruses is important for obtain data due to emerging and re-emerging arboviruses. Iran has been known to potent transmission of arboviruses such as West Nile virus (WNV) and dengue virus (DENV) based on entomology and sero epidemiological data.Objectives: This study was done to obtain the data about the potential mosquito vectors forarboviralflaviviruses.Materialsand Methods:Culicidae mosquitoes were collected at three fixed and five variable stations sites in Dorood area, Lorestan province, western Iran in 2017. Mosquito’s adults were collected using human baited bed net trap hand catch, and larvae by dipping methods.RT-PCR for detection of flaviviruses was performed.Results: In total, 11 species were collected and were morphologically identified including; Culexperexiguus, Cx.pipiens, Cx.theileri, Cx.mimiticus, Cx.modestus, Cx.hortensis, Culisetalongiareolata, Anopheles dthali, Anstephensi, An.claviger, An.sacharovi. Culexpipiens (53.21%)was found dominant species. The mosquitoes were distributed into 22 pools, analyzed and found negative for flavivirus RNA.Conclusion:It is the first formal entomological research which defines the screening of the possible arboviral infection of mosquitoes in Dorood area of Iran. There was no detection for arboviral infection. Periodic interval mosquito-based arbovirus surveillance using morphological and molecular detection techniques could be still suggested

Keywords:

Mosquitoes , Arboviral infection , Dorood area , Iran