The anti- proliferative effects of Ferulago angulata on human promyelocytic leukemia cell line (HL-60)

Aim: Ferulago angulata is a medicinal plant with bioactive compounds having antioxidant activities. F.angulata extract can be a good candidate as chemo- preventive agent against cancer cells. In this study, the pro-apoptotic activities of F.angulata were investigated on human promyelocytic leukemia cell line (HL-60). Material and Methods: HL-60 cell line was cultured in RPMI 1640 medium and then treated with different concentrations of F.angulata extracts. The effect of extract on cell viability was measured by MTS assay. The apoptosis was evaluated using flowcytometry. IC50 was calculated by probit analysis and the groups were compared using Kruscal wallis test.Result: Our results show that F.angulata decreased cell viability in a concentration of more than 500 µg/ml with an IC5 of about 1000 µg/ml. Apoptosis was observed in all concentrations, but only was remarkable in more than 500 µg/ml. Conclusion: F.angulata can induce anti-proliferative activities against HL-60 cells. A complete understanding of molecular events and pharmacokinetics of the elements and clinical trials in animal models are required for dose determination and its interaction with other components of combination chemotherapy.

Keywords:

MTS , apoptosis , HL-60 cell line , Ferulago angulata