Association between four novel miRNAs polymorphisms and susceptibility to idiopathic recurrent spontaneous abortion

Background and Aim : A growing body of evidence suggests that microRNAs play fundamental regulatory roles in embryo implantation and maintenance of pregnancy. The aim of this study was to investigate the possible association between miR-146a C> G, miR-149 T> C, miR-196a2 T> C and miR-499 A> G polymorphisms and genetic susceptibility to Recurrent Spontaneous Abortion (RSA). Methods : One hundred and twenty women with a history of two or more unexplained consecutive miscarriages and ninety ethnically-matched healthy women with a history of at least two successful pregnancy outcomes and without a history of miscarriage were enrolled in a case-control study. Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results : Our findings showed that the prevalence of miR-149 T> C polymorphism in RSA patients was significantly higher than those in healthy controls (p < 0.05). We also found that the presence of miR-149 C and miR-499 G alleles was significantly associated with susceptibility to RSA (p < 0.05). The miR-146a CC /miR-499 GG, miR-149 TC /miR-499 AG and miR-196a2 TT /miR-499 GG combined Genotypes were associated with the high risk of RSA(p < 0.05). Conclusion : This study suggests that miR-149 T> C polymorphism and the presence of miR-149 C, and miR-499 G alleles are a genetic determinant for the risk of idiopathic RSA.

Keywords:

miR-146a C> G , miR-149 T> C , miR-196a2 T> C , and miR-499 A> G , Recurrent Spontaneous Abortion (RSA)