Alteration of NR2A and NR3A genes expression in Morphine addicted male rat following Crocin administration in Hippocampus and Striatum regions

Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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NSCMED08_164

تاریخ نمایه سازی: 15 دی 1398

Abstract:

Background and Aim : Addiction is a disorder of the brain s reward system which arises through transcriptional and epigenetic mechanisms and occurs over time from chronically high levels of exposure to an addictive stimulus. Morphine is a pain medication of the opiate variety which is found naturally in a number of plants and animals. It acts directly on the central nervous system (CNS) to decrease the feeling of pain. Morphine is a highly addictive substance. Neurodegenerative drugs like crocin is currently used as drug for the restoration of cerebral ischemia. The aim of this study was to evaluate the NR2A and NR3A gene expression in striatum and hippocampus and assess the Crocin capacity as the neuroprotection agent.Methods : All experiments were performed on male rats obtained from Pasteur Institute weighing 220-240g at the beginning of the experiment. They were housed in groups of five in each cage and they were maintained under the controlled environmental conditions. A total of 20 rats were divided into 4 groups: control, morphine, morphine-Crocin and Crocin. In the Crocin group, Crocin administration was performed every other day through the 21-day morphine addiction procedure. All the groups were treated for 21 consecutive days and following the drug treatment, dissected part of the brain was removed on day 21 for the control, morphine and Crocin groups. Following the decapitation, different parts of the brain including hippocampus and Striatum regions were removed from the skull and the regions were dissected on a plate full of ice. The samples were immediately lysed and the RNA extraction procedure was carried out. The expression of NR2A and NR3A was evaluated by performing Real time PCR. The statistical analysis was performed with one-way ANOVA and t-test method.Results : From t-test analysis, it can be concluded that injection of morphine significantly affects the expression of NR2A gene in the hippocampus (P <0.01) but does not have a significant effect on the gene in the striatum (P> 0.05 ) The expression of NR3A gene was meaningless in the hippocampus and striatum (P> 0.05, P> 0.05, respectively). In addition, Crocin has a significant effect on the increase of NR2A expression in the hippocampus compared to morphine (P <0.05), but has no significant effect on striatum (P> 0.05). This drug has no significant effect on the expression of NR3A gene in the hippocampus But it reduced the expression of NR3A gene in the striatum (P> 0.05 and P <0.01). Conclusion : Taking together, all the findings suggested that the NMDA main subunits does change significantly following the Morphine uptake in rats in Hippocamus and Striatum regions. Moreover, we did find Crocin as a neuroprotection agent on responses induced by morphine.

Authors

Tina Eshaghian

Cognitive and Neuroscience Research Center, Amir al Momenin Hospital, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Mohammad Nasehi

Cognitive and Neuroscience Research Center, Amir al Momenin Hospital, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Shahram Zarabian

Cognitive and Neuroscience Research Center, Amir al Momenin Hospital, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran