Effect of Reelin protein on endogenous neural progenitor cell migration and differentiation in animal model of stroke

Background and Aim : This study investigated the ability of intra-subventricular zone (SVZ) administration of Reelin protein on neural progenitor cells (NPCs) attraction from the SVZ toward the infarct cavity in photothrombotic stroke model of mice.Methods : Bulb-c mice were assigned to four groups of the sham, stroke, and stroke+Reelin. The animal model of stroke was induced by green laser in right hemisphere under Stereotaxy. mice in the treatment groups received an intra SVZ injection of Reelin or the vehicle of Reelin one week after induction of stroke and were then subjected to the mNSS test on days 4 and 25 after Reelin injection. Bromodeoxyuridine (BrdU) was intraperitoneally injected to label newly generated cells. The samples were collected from the distance between SVZ and infarct cavity. Differentiation and proliferation of NPCs were assessed by BrdU/nestin/neuN double immunofluorescence method on days 5 and 28 after Reelin infusion.Results : Stroke induced mice showed decreased neurological function compared to the Sham group. However, administration of Reelin improved motor and sensory function and the number of neuN expressing neuroblasts in the SVZ as compared to the stroke and stroke+Vehicle groups.Conclusion : These results suggest that Reelin enhances the proliferation and differentiation of neural stem cells (NSCs) migrating toward the infarct cavity accompanied by improvement of stroke-induced neurological dysfunctions.

Keywords:

Subventricular Zone , Reelin , photothrombotic stroke