Evaluation of the expression level of PINT Long none coding RNA in tissue samples of patients with colorectal cancer

Introduction: Colorectal cancer (CRC) is commonly diagnosed in advanced stages with poor prognosis so development and using of biomarkers such as long non coding RNA to detect at early stages and reduce cancer-related mortality is recommended. Long non-coding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides that have a main role in regulation of gene expression. In this study we evaluated the expression level of PINT Lnc RNA, main regulator of the P53 gene expression, in the tissue samples of CRC patients and compared with clinical characterizations.Materials and Methods: In this study, 50 CRC tissue samples with pair normal were collected from surgery department of Taleghani hospital, Tehran, Iran. Lnc RNA PINT expression was evaluated using Real Time PCR method. Fold change of gene expression was evaluated by (2-∆∆ Ct) method. Results: Down regulation of mRNA expression was found in PINT level in tissue samples of CRC patients comparing to pair normal. These changes were linked with patient’s TNM stage (p˂0.05).Conclusion: The results of this study indicate that evaluation of PINT LncRNA expression in the tissue sample can be used as a biomarker for early diagnosis of colorectal cancer.

Keywords:

PINT Lnc RNA , Colorectal cancer , early diagnosis , TNM