The Effect of Fetal Bovine Serum on Differentiation of Ovine Myogenic Satellite Cells

Satellite cells are a population of adult muscle stem cells that play a key role in mediating muscle regeneration. The established muscle-derived satellite cells model can be used to study the genes associated with muscle development, and as seed cells for animal biotechnology related studies. Sheep satellite cells have a greater similarity to human satellite cells with regard to metabolism, life span, proliferation and differentiation, than satellite cells of the rat and mouse; from this feature, can be used as an animal model for the treatment of human diseases. These cells are precursors of myoblast cells. The purpose of this study was to determine the effect of the Fetal Bovine Serum (FBS) content on the process of satellite cells growth rate and differentiation. The satellite cells were isolated from Semimembranosus and Semitendinosus Muscle tissues of 50 to 60-day-old Kurdi sheep fetuses. After Enzymatic digestion, a combination of satellite and non-myogenic cells was cultured on the flask. Flasks were replaced after 3 hours to isolate non-myogenic cells, such as fibroblasts. After 6 days, the cells differentiated. Then the cell growth were evaluated by counting for 8 continuous days in media containing 0, 5 and 10% of FBS. The analysis of cell growth showed that the differentiation of satellite cells was significantly higher in enriched medium with 10% FBS (P<0.05).

Keywords:

Satellite cells , Sheep , Fetal bovine serum , Differentiation.