Effects of PARP-1 762 Codon polymorphism on Breast Cancer Risk among Iranian Population

Breast cancer is a heterogeneous and hormone dependent cancer that includes about 22.9% of total female cancers. It is the most common type diagnosed cancer and the fifth cause of cancer death among women. DNA repair pathways play important roles in maintaining genomic stability and influence carcinogenesis and tumor biology. Poly [ADP-Ribose] polymerase (PARP) is a key DNA repair enzyme which is essential for DNA single-strand break (SSB) repair -a sub-pathway related to base excision Repair. PARPs proteins also have been involved in various cellular processes including cell survival and death, transcriptional and chromatin structure regulations, telomere integrity, and cell division. Several studies have demonstrated the association of PARP-1 gene polymorphisms with the incidence risk of breast cancer. We analyzed the status of an active-site polymorphism (rs1136410) in the PARP-1 gene and their possible impact with predisposing individuals to breast cancer disease in a female Iran population. A total of 100 patients with histopathologically and surgically confirmed breast cancer and 100 controls was recruited. Genomic DNA was extracted from peripheral blood leukocytes through Salting Out standard technique. Genotyping was performed using ARMS-PCR protocol. There was no significant difference between the PARP-1 V762A polymorphism distribution in control and patient groups. The PARP-1 V762A seems not to be a potential risk factor for the incidence of breast cancer among Iranian patients.