Toxicity of multi-wall carbon nano tubes inhalation on the brain of rats
Publish place: 15th iranian congress of toxicology
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
TOXICOLOGY15_048
تاریخ نمایه سازی: 15 بهمن 1398
Abstract:
This study was designed to investigate the brain toxicity following the respiratory contact with multi-wall carbon nanotubes (MWCNTs) in male Wistar rats. Rats were exposed to 5 mg/m3 MWCNT aerosol in different size and purity for 5 hours/day, 5 days/week for 2 weeks in a whole-body exposure chamber. After 2-weeks exposure, mitochondrial isolation were performed from different parts of rat brain (hippocampus, frontal cortex, and cerebellum) and parameters of mitochondrial toxicity including Mitochondrial succinate dehydrogenase (SDH) activity, generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, Mitochondrial swelling, and cytochrome c release, ATP level, mitochondrial GSH and lipid peroxidation, were evaluated. Our results demonstrated that MWCNTs with different characteristics, in size and purity, significantly (P<0.05) decreased SDH activity, GSH, and ATP level, increased mitochondrial ROS production, lipid peroxidation, mitochondrial swelling, MMP collapse, and cytochrome c release in the brain mitochondria. In conclusion, we suggested that MWCNTs with different characteristics, in size and purity, induce damage in varying degrees on the mitochondrial respiratory chain and increase mitochondrial ROS formation in different parts of rat brain (hippocampus, frontal cortex, and cerebellum).
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Authors
Fatemeh Samiei
Department of Pharmacology and Toxicology, Faculty of Pharmacy, ShahidBeheshtiUniversity of Medical Sciences
Farshad Hosseini Shirazi
Department of Pharmacology and Toxicology, Faculty of Pharmacy, ShahidBeheshtiUniversity of Medical Sciences- Pharmaceutical Sciences Research Center, ShahidBeheshti University of Medical Sciences, Tehran, Iran
Jalal Pourahmad
Department of Pharmacology and Toxicology, Faculty of Pharmacy, ShahidBeheshtiUniversity of Medical Sciences