Serotyping and Antimicrobial Susceptibility Pattern of Escherichia coli Isolates from Urinary Tract Infections in kidney transplant patients

Introduction and Objectives: Urinary tract infections (UTIs) are the most common infections in renal transplant recipients and are considered as a potential cause of bacteremia, sepsis, and affects graft outcomes(1). The aim of this study was to isolate and serotype and the E. coli isolates in kidney transplant patients with urinary tract infections (UTIs) and to assess their antibiotic susceptibility patterns. Materials and Methods: In this study, 53 E. coli isolates were collected from Kidney patients of Labbafinejad hospital and Yekta Labs during Apr to May 2019. All isolates were reconfirmed by standard bacteriologic methods and kept in 10% glycerol +TSB at -70°C. Antimicrobial susceptibility test (AST) was done according to CLSI 2018 protocol. Serotyping of E. coli isolates was identified by slide agglutionation method and using BaharAfshan antiserum kit. Results: Only 50 isolates reconfirmed as E. coli after biochemichal tests and 3 samples were excluded of this study. By slide agglutination, the frequency of serotypes were as: 25 isolates serotyping I (O126,O55,O111) and 6 samples serotyping II (O86,O127), 12 samples serotyping III (O44,O125,O128) and 4 samples serotyping VI (O120,O114).. Based on AST, highest antibiotic susceptibility were to: doripenem, fosfomycine and ertapenem (100%), Imipenem 95%, also, the highest antibiotic resistance were to: ampicillin (86%), cefotaxime (80%), cefazolin and cefpodoxime (77%). Conclusion: Because of high resistant rate to common antibiotics based on AST results and critical effect of accurate antibiotic prescription on the graft’s destiny among Kidney transplant patients, smart local screening and doing AST before any prescription should be mandatory.

Keywords:

E. coli , drug resistance , kidney transplantation