Optimizing the Immobilization of Biosurfactant-Producing Pseudomonas aeruginosa in Alginate Beads
Publish place: 20th International Congress of Microbiology of Iran
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM20_146
تاریخ نمایه سازی: 26 بهمن 1398
Abstract:
Introduction and Objectives: optimizing the immobilization of pseudomonas aeruginosa as a biosurfactant producer was studied. Materials and Methods: Biosurfactant production by p. aeruginosa was confirmed through the hemolysis test, emulsification index (EI), and surface activity measurement. Calcium alginate encapsulation technique was used in order to entrap the P. aeruginosa cells. Full factorial design was employed to optimize bead preparation. Furthermore, the morphology and the stability of beads were evaluated. Results: It was proved that immobilized cells can be preserved the viability and biosurfactant production. Application of full factorial design indicated that the values of three parameters sodium alginate 3 %, CaCl2 1 % (w/v), and hardening time of 35 min, was found to be too stable for minimum surface tension and stable alginate gel beads. Conclusion: Finally, it could be concluded that the alginate gel beads showed good stability during the growing process and the immobilized cells efficiently were viable. Alginate source, hardening time and the interaction between CaCl2 concentration and hardening time influenced on the bead preparation.
Keywords:
Authors
Mandana Ohadi
Pharamceutics Research Center, Kerman University of Medical Sciences
Gholamreza Dehghannoudeh
Pharamceutics Research Center, Kerman University of Medical Sciences
Kioomars Kiani
Pharamceutics Research Center, Kerman University of Medical Sciences
Mohammad Hassan Moshafi
Pharamceutics Research Center, Kerman University of Medical Sciences
Negar Dehghannoudeh
Pharamceutics Research Center, Kerman University of Medical Sciences