Application of the more affordable nanoparticles against the main bacterial infectious diseases in rainbow trout rearing industry
Publish place: 20th International Congress of Microbiology of Iran
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
View: 344
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
MEDISM20_306
تاریخ نمایه سازی: 26 بهمن 1398
Abstract:
Introduction and Objectives: Emerging of Infectious outbreaks caused by bacterial agents in the aquaculture industry, expansion of bacterial resistance to antimicrobial agents, and trouble of drug residuals in the products have led to the application of novel, safe and affordable alternatives such as Nanoparticles (NPs). The present study intends assessing the toxicity of CuO and ZnO NPs at In vitro conditions on the main bacterial pathogenic agents for the reared fish, including Lactococcus garvieae, Streptococcus iniae, and Yersinia ruckeri. Material & Methods: First, the physical features of NPs crystals were determined by X-ray diffraction (Shimadzu, XRD-6100). Then, the sensitivity of them to the mentioned NPs was assayed with compared florfenicol, as a reference antibiotic, through the well diffusion method. Also, MIC/MBC were determined by the microdilution technique. We use one-way ANOVA in order to data analysis. Results: The diameter of Nano CuO and ZnO crystals was estimated 41.48 and 49.6 nm, respectively. Evidence indicates the positive gram bacteria had intermediate sensitivity to ZnO, but they were resistant to CuO. However, enteric redmouth disease agent was absolutely sensitive to ZnO. Nano zinc oxide and copper oxide could significantly inhibit the growth of Streptococcus iniae or kill it at 0.18 and 0.24 μg/ml and more, respectively. Also, MIC/ MBC of ZnO NP were obtained for Lactococcus garvieae and Yersinia ruckeri, in order equal to 0.18 and 0.06 μg/ml. Conclusion: Antibacterial potency of Nano ZnO was more than another NP and florfenicol at same concentration. Therefore, the application of it will be advised if there are not the observable toxic effects of this NP at the suggested concentration for reared fish.
Keywords:
Nano copper oxide (CuO) , Nano zinc oxide (ZnO) , Lactococcus garvieae , Streptococcus iniae , Yersinia ruckeri.
Authors
Nima Shiry
Division of Aquatic Animal Health, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;
Amin Gholamhosseini
Division of Aquatic Animal Health, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;
Mostafa Akhlaghi
Division of Aquatic Animal Health, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;