Propolis ethanol extract effect on the cytoplasmic membrane in Yersinia ruckeri causative agent of salmonids’ enteric red mouth disease

Introduction and Objectives: Propolis is a resinous substance produced by honeybees which collected from different plants. Yersinia ruckeri is a gram-negative stain bacilli and the causative agent of salmonids’ enteric red mouth disease. The aim of this article is to identify the effect mechanism of propolis ethanol extract on the cytoplasmic membrane in Yersinia ruckeri. Materials and Methods: After collecting propolis from beehives, propolis ethanol extract was prepared; and analysis of constituent materials was carried out by gas chromatography-mass spectrometry (GC-MS) method. Then propolis ethanol extracts antibacterial activity against Yersinia ruckeri Was performed by microdilution technique. In the next step, bacteria face with the 1× concentration of inhibitory concentration in growth level and rate of ATP production, membrane potential and the ratio of NAD+NADH⁄ the rate was measured. statistical survey of data was done with the analysis of variance (ANOVA) method. Results: Results showed the minimum bacteriostatic and bactericidal dose of propolis ethanol extract against Yersinia ruckeri equal 62.5 and 125 μgml⁄ by sequence; and ATP production rate and Yersinia ruckeri membrane potential in effect of propolis ethanol extract were significantly reduced. Conclusion: Based on the results, it can be concluded propolis ethanol extract applies its antibacterial action in Yersinia ruckeri by inhibiting peptidoglycan synthesis and cytoplasmic dysfunction.

Keywords:

Propolis , salmonid , Yersinia ruckeri