Evaluation of Antifungal effects of TiO2 and Amphotericin B on different Candida species in Vitro condition

Introduction and Objectives: Candida species are known as cause serious fungal infections that produce cutaneous, mucosal and systemic infections. Nowadays, mortality and morbidity due to Candida species especially in immunocompromised patients was increased. Therefore, finding new approach to treat and control of these infectious agents necessary. Nanotechnology is science, engineering, and technology conducted at the nanoscale, which is about 1 to 100 nanometers. The aim of this study was to evaluate the antifungal effects of titanium dioxide nanoparticles and Amphotericin B on different Candida spp. Materials and Methods: In present study, Minimum Laboratory Concentration (MIC) of titanium dioxide Nano particles and Amphotericin B were determined by broth microdilution (BMD) methods according to the guideline of Clinical and Laboratory Standard Institute (CLSI) (M27-A3 document). In Addition, Minimum fungicidal concentrations (MFC) was defined as the lowest concentration of the TiO2NPs and or AmB that killed 98%-99.9% of each Candida spp. Results: The results showed that the TiO2-NPs had antifungal activity against pathogenic Candida spp. and could inhibit the growth of all the tested Candida spp. The MIC and MFC of TiO2NPs against Candida spp. was 128 to 256 μg/ml and 256 to 512 μg/ml, respectively. MIC and MFC of AmB were 8 to 16 μg/ml and 16 to 32 μg/ml, respectively. Conclusion: Our finding showed that the TiO2 had antifungal potential against pathogenic Candida spp. and could inhibit the growth of all tasted Candida spp. However, their antifungal properties was significantly less than AmB.

Keywords:

Candida spp , TiO2 Nanoparticles , Amphotericin B.