A novel sorbent based on metal–organic framework for mercury separation from human serum samples by ultrasound assisted- ionic liquid-solid phase microextraction

In this research, the metal–organic framework (MOF) as a solid phase was used for separation mercury [Hg (II)] inhuman serum sample by ultrasound assisted- Ionic Liquid-solid phase microextraction procedure (USA- IL-μ-SPE). Mercury extracted from serum sample by [Zn2(BDC)2(DABCO)]n as MOF at pH=8. Hydrophobic ionic liquid ([BMIM] [PF6]) was used as solvent trap for Hg-MOF-NC from the sample solution. The phase of Hg- MOF-NC was back extracted by 0.5 mL of HNO3 (0.2 mol L-1) and finally mercury concentration determined with cold vaporatomic absorption spectrometry (CV-AAS) after dilution with 0.5 mL of DW. Under the optimal conditions, the linear range, limitof detection and preconcentration factor were obtained 0.02– 5.5 μg L−1, 6.5 ng L−1 and 9.8 for serum samples, respectively (%RSD<5%). The validation of methodology was confirmed by standard reference materials (SRM).

Keywords:

Metal–organic framework , Ultrasound assisted -micro-solid phase extraction , Mercury , Serum samples , Cold vapor atomic absorption spectrometry