Real Time PCR of MBP after After Mesenchymal Stem cell Injection in Cuprizone Model of Multiple Sclerosis.
Publish Year: 1398
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
HUMS01_071
تاریخ نمایه سازی: 6 اسفند 1398
Abstract:
Objectives.Multiple sclerosis (MS) is one of the most important inflammatory diseases of the central nervous system, yet its etiology is not well defined. Cell therapy for MS has become one of the newest therapies, even in its clinical phase. Material and Method First, human bone marrow stromal stem cells are cultured with α-MEM containing 10% FBS. Demyelination model was induced in mice for 6 weeks by administration of the cuprizone, and in the fourth and fifth week after the first dose of Cuprizone, the stem cells were administered intraperitoneally to the mice. At the end of the sixth week, the rats were killed and the expression of MBP was investigated by realTime-PCR. .Results In the molecular studies, significantly increase the expression of the MBP in the treatment group with the cell was seen compared to other groups.Conclusion In treatment we observed an increase in myelination compared to other groups that we used only from PBS.
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Authors
Vahid Ghanbarzehi
Research Committee, Iranshahr University of Medical Sciences, Iranshahr,Iran (I.R.Iran)
Mohsen Marzban
Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Maryam Kadkhodazadeh
Research Committee, Iranshahr University of Medical Sciences, Iranshahr,Iran (I.R.Iran)
Fatemeh Esmaeili
Research Committee, Iranshahr University of Medical Sciences, Iranshahr,Iran (I.R.Iran)
Behzad Notizehi
Research Committee, Iranshahr University of Medical Sciences, Iranshahr,Iran (I.R.Iran)
Alireza Garkhil
Research Committee, Iranshahr University of Medical Sciences, Iranshahr,Iran (I.R.Iran)