Association of Antioxidant Enzyme Leveland Sperm Parameters in Fertile And Infertile Men

Backgournd: ReactiveOxidative stress (ROS) affects fertility and influences on the sperm quality, a molecular marker of stress related infertility. In the current study, we aimed to assess the TAC (total antioxidant capacity) and MDA (malondialdehyde) as stress oxidative markers in infertile men with Teratoastenozospermia and fertile men, and moreover, their correlation with sperm parameters. Methods: The study was carried out inUnit of Infertility Research Center of the ACECR, Qom.Iran,This case control study was performed on 25 infertile men with Teratoastenozospermia and 25 men with proven fertility.The semen analysis waspreformed according to WHO criteria.The ferric reducing ability of plasma (FRAP) and thiobarbituric acid (TBA) reaction methods were used for seminal plasma TAC and MDA assay, respectively. Results: Sperm Pregressive motility (a+b) (%) (28,94±19,93 vs. 37,59±16,44, p<0,04) and sperm morphology (%) (4,10±3,21 vs. 5,57±5, 64,P< 0.04) were observed in infertile men with Teratoastenozospermia compared to fertile men.Lower TAC levels (1.7±0.2 vs. 1.3±0.4 mmol/L, P=0.0004) and higher MDAlevels (2.5±1.1 vs. 5.8±1.9 mmol/L, P < 0.0001) wereshowed a negative correlation was found betweenTAC and MDA levels in infertile men. Conclusions: Decreased antioxidant enzyme levels(TAC) are associated with infertility. The MAD levels were significantly higher inpatients with abnormal semen parameters. High degree of correlation betweensperm parameters and antioxidant enzymes suggests the ability ofabnormal spermatozoa to produce reactive oxygen species resulting inreduced levels of antioxidant enzyme

Keywords:

Reactive Oxygen Species (ROS) , Total Antioxidant Capacity(TAC) , Malondialdehyde (MDA) , Infertility