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Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS

Publish place: International Journal of Horticultural Science and Technology، Vol: 6، Issue: 2
Publish Year: 1398
Type: Journal paper
Language: English
View: 610

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Link to this Paper:
https://civilica.com/doc/1007589

Document National Code:

JR_IJHST-6-2_001

Index date: 10 April 2020

Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS abstract

Narcissus spp. are of great importance to pharmaceutical industries because of biosynthesis of Amaryllidaceae alkaloids. N. tazetta with several varieties is a widespread species, native to the Mediterranean region with considerable distribution in Iran. In the present study, calli, roots and bulblets were regenerated from N. tazetta var. Meskin. In order to report alkaloid profile and quantify galanthamine and lycorine, methanolic extract of different in vitro tissues were subjected to alkaloid purification by SPE (solid phase extraction) method, followed by GC-MS analysis. In general, galanthamine and lycorine were detected in all in vitro tissues, while demethylmaritidine was only identified in bulblets. Bulblet was identified as the best source of main Amaryllidaceae alkaloids regarding the highest quantities of galanthamine and lycorine with 15 and 731 µg/g dry weight (DW) respectively.

Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS Keywords:

Amaryllidaceae alkaloids , Galanthamine , Lycorine , Narcissus , Tissue culture

Quantitative Determination of Lycorine and Galanthamine in Different in Vitro Tissues of Narcissus tazetta by GC-MS authors

Ameneh Tarakemeh

Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad, Iran.

Majid Azizi

Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad, Iran.

Vahid Rowshan

Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran.

Hassan Salehi

Department of Horticultural Science, Shiraz University, Shiraz, Iran.

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