PI3K/Akt/mTOR and CDK4 combined inhibition enhanced apoptosis of thyroid cancer cell lines
Publish place: International journal of Advanced Biological and Biomedical Research، Vol: 8، Issue: 2
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJABBR-8-2_010
تاریخ نمایه سازی: 23 تیر 1399
Abstract:
Introduction Thyroid cancer is a malignant disease with poor prognosis. The PI3K/Akt/mTOR and Cyclin-Dependent Kinase 4 (CDK4) pathways are vital regulators of tumor cell proliferation and survival. Therefore the present study was designed to use dual inhibition of such pathways to kill thyroid cancer cells. Methods and materials The effects of each inhibitors on human ATC and BCPAP cell lines were evaluated by MTT assay. The suitable concentrations of inhibitors were determined and synergistic effects of such inhibitors were evaluated by bax/bcl-2 mRNA ratio, Caspase-3, and Caspase-9 activity assay as well as Akt, mTOR, and CDK4. Results:Our finding showed that both ATC and BCPAP cell proliferation is significantly inhibited by PD-332991(PD) and NVP-BEZ235 (NVP) in a time and concentration-dependent manner (P
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Authors
Neda Hamidipour
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mehdi Fazeli
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mehdi Hedayati
۲. Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Mohsen Dehghani
Rayan Pars Pharmacutical Industry, shiraz, Iran
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