بررسی در مورد تولید انبوه سلول های لاین آلوده به شیزونت تیلریا آنولاتا در سیستم بیوراکتور هوازی
صاحب اثر: سازمان تحقیقات، آموزش و ترویج کشاورزی
نوع محتوی: طرح پژوهشی
Language: Persian
استان موضوع گزارش: البرز
شهر موضوع گزارش: کرج
Document ID: R-1061703
Publish: 16 February 2019
دسته بندی علمی: علوم کشاورزی
View: 264
Pages: 21
Publish Year: 1388
نسخه کامل Research منتشر نشده است و در دسترس نیست.
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استخراج به نرم افزارهای پژوهشی:
Abstract:
After the first project from a series of , in production of bovine theileriosis vaccine in aerobic bioreactor, this is the second project that reviews the conditions of multiplication and cell growth during the cell culture for production of this vaccine . As the bioreactor was not prepared for the investigator anytime needed, firstly some experiments were carried out to understand the problems and know the critical points. First , liters vessels were used for cell culture , and after that, the biomixer, a mechanical agitation vessel with entrance canals for medium or for instruments, was used and some acceptable results gained. Then the bioreactor of liters, from FMD department was used to perform the project protocol and get the final results. According to the results, when the start cell population in the culture is appropriate, the final population will be times greater than flask method and the pH of medium and the level of diluted Oxygen in the medium are the most important factors, after that
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