Isolation of broad-host-range bacteriophages against food- and patient-derived Shiga toxin-producing Escherichia coli
Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
View: 240
This Paper With 10 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJVST-12-1_002
تاریخ نمایه سازی: 17 دی 1399
Abstract:
This study aimed to isolate bacteriophages specific to Shiga toxin-producing Escherichia coli (E. coli) strains, particularly EHEC O157:H7, in order to develop a collection of phages against different E. coli pathotypes isolated from northeast of Iran. Eighteen samples were screened without any preliminary enrichment and also with small scale enrichment using E. coli 12900, which did not result in the phage recovery. Seven samples were prepared with an extensive enrichment. Of them, 5 samples produced plaques. Eventually, seven phages out of thirteen isolated phages were selected for phage host range investigation. Results of the spotting host range assay demonstrated that 22 pathogenic E. coli strains and isolates (54%) were susceptible to at least one of the phages. Phage Ecol-MHD1 was polyvalent against E. coli and Salmonella isolates. The other phages were specific to E. coli pathotypes. In conclusion, the phages isolated in this study can be suggested as preventive or therapeutic candidates against foodborne E. coli infections in humans.
Keywords:
Authors
Golshan Shakeri
Ferdowsi University of Mashhad
Abdollah Jamshidi
Department of Faculty of Veterinary Medicine
Kiarash Ghazvini
Mashhad university of medical sciences
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :