Investigating the expressions of miRNA-125b and TP53 in endometriosis. Does it underlie cancer-like features of endometriosis? A case-control study

Publish Year: 1399
نوع سند: مقاله ژورنالی
زبان: English
View: 168

This Paper With 12 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

این Paper در بخشهای موضوعی زیر دسته بندی شده است:

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_IJRM-18-10_002

تاریخ نمایه سازی: 17 فروردین 1400

Abstract:

Background: Endometriosis is generally considered as a benign condition; however, there is a possibility for it to become cancerous. miR-125b is upregulated in both endometriotic tissues and serum samples of women with endometriosis but its potential targets in endometriosis are still not fully understood. Objective: The role of miR-125b in the regulation of TP53 expression in endometriosis was tested with a bioinformatics approach. In addition, the expression of miR-125b and TP53 in both eutopic (Eu-p) and ectopic endometrium (Ec-p) in the endometrium tissues of women with endometriosis was compared to those in the normal endometrium tissues of controls (Normal). Materials and Methods: In this case-control study, the Eu-p and Ec-p samples were collected from 20 women who underwent laparoscopic surgery, and the normal endometrium tissues were collected from 20 controls with no evidence of endometriosis. For bioinformatics approach, a protein-protein interaction network was constructed based on co-expressed potential targets of miR-125b. Quantitative polymerase chain reaction technique was used for the measurement of miR125b and TP53 expression. Results: Our results showed that miR-125b was significantly overexpressed in Ec-p (p-value: 0.021). In addition, there was a significant TP53 under expression in both the Ec-p and Eu-p samples compared with the Normal tissues (p-value: 0.003). Conclusion: The negative correlation between miR-125b and TP53 as well as a noticeable decreased expression of TP53 in both Ec-p and Eu-p samples may be interpreted as the roles of miR-125b/TP53 axis in the pathogenesis of endometriosis. In addition, these findings and bioinformatic analyses imply a possible role of miR-125b in cancer-like features of endometriosis.

Authors

Elnaz Hajimaghsoudi

Department of Genetics, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Farzaneh Darbeheshti

Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Breast Cancer Association (BrCA), Universal Scientific Education and Research Network (USERN), Tehran, Iran.

Seyed Mehdi Kalantar

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Atiyeh Javaheri

Department of Obstetrics and Gynecology, Faculty of Medicine, Shahid Sadoughi Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Seyed Hamidreza Mirabutalebi

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Student Research Committee, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Mohammad Hasan Sheikhha

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Halis G, Arici A. Endometriosis and inflammation in infertility. Ann ...
  • Sampson JA. Peritoneal endometriosis due to the menstrual dissemination of ...
  • Munksgaard PS, Blaakaer J. The association between endometriosis and ovarian ...
  • Mogensen JB, Kjær SK, Mellemkjær L, Jensen A. Endometriosis and ...
  • Yang HP, Meeker A, Guido R, Gunter MJ, Huang GS, ...
  • Ishii M, Yamamoto M, Tanaka K, Asakuma M, Masubuchi S, ...
  • Mann S, Patel P, Matthews CM, Pinto K, O'Connor J. ...
  • Kao LC, Germeyer A, Tulac S, Lobo S, Yang JP, ...
  • Wu Y, Kajdacsy-Balla A, Strawn E, Basir Z, Halverson G, ...
  • Hever A, Roth RB, Hevezi P, Marin ME, Acosta JA, ...
  • Arimoto T, Katagiri T, Oda K, Tsunoda T, Yasugi T, ...
  • Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; ...
  • Tang W, Jiang Y, Mu X, Xu L, Cheng W, ...
  • Xu P, Guo M, Hay BA. MicroRNAs and the regulation ...
  • Cheng AM, Byrom MW, Shelton J, Ford LP. Antisense inhibition ...
  • Etheridge A, Lee I, Hood L, Galas D, Wang K. ...
  • Ghasemi N, Karimi-Zarchi M, Mortazavi-Zadeh MR, Atash-Afza A. Evaluation of ...
  • Sun YM, Lin KY, Chen YQ. Diverse functions of miR-125 ...
  • Ohlsson Teague EMC, Van der Hoek KH, Van der Hoek ...
  • Cosar E, Mamillapalli R, Ersoy GS, Cho S, Seifer B, ...
  • Chang CYY, Chen Y, Lai MT, Chang HW, Cheng J, ...
  • Ohlsson Teague EMC, Print CG, Hull ML. The role of ...
  • Le MTN, Teh C, Shyh-Chang N, Xie H, Zhou B, ...
  • Yang H, Kang K, Cheng C, Mamillapalli R, Taylor HS. ...
  • Allavena G, Carrarelli P, Del Bello B, Luisi S, Petraglia ...
  • Gloria-Bottini F, Ammendola M, Saccucci P, Neri A, Magrini A, ...
  • Lao X, Chen Z, Qin A. p53 Arg72Pro polymorphism confers ...
  • Mirabutalebi SH, Karami N, Montazeri F, Fesahat F, Sheikhha MH, ...
  • Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, ...
  • Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, ...
  • Morris JH, Apeltsin L, Newman AM, Baumbach J, Wittkop T, ...
  • Kuleshov MV, Jones MR, Rouillard AD, Fernandez NF, Duan Q, ...
  • Vlachos IS, Zagganas K, Paraskevopoulou MD, Georgakilas G, Karagkouni D, ...
  • Gemmill JAL, Stratton P, Cleary SD, Ballweg ML, Sinaii N. ...
  • Calin GA, Croce CM. MicroRNA signatures in human cancers. Nat ...
  • Razi MH, Eftekhar M, Ghasemi N, Sheikhha MH, Dehghani Firoozabadi ...
  • Hawkins SM, Creighton CJ, Han DY, Zariff A, Anderson ML, ...
  • Zhou M, Liu Z, Zhao Y, Ding Y, Liu H, ...
  • Bousquet M, Harris MH, Zhou B, Lodish HF. MicroRNA miR-125b ...
  • Xia HF, He TZ, Liu CM, Cui Y, Song PP, ...
  • Bousquet M, Nguyen D, Chen C, Shields L, Lodish HF. ...
  • Nagpal V, Rai R, Place AT, Murphy SB, Verma SK, ...
  • https://doi.org/10.1161/CIRCULATIONAHA.115.018174 ...
  • Jiang L, Huang Q, Chang J, Wang E, Qiu X. ...
  • Wang Y, Zhang Y, Kong C, Zhang Z, Zhu Y. ...
  • Neilsen PM, Noll JE, Mattiske S, Bracken CP, Gregory PA, ...
  • Kao AP, Wang KH, Chang CC, Lee JN, Long CY, ...
  • نمایش کامل مراجع