Evaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers

Recognition of genetic reserves and desirable genes is the basis of breeding programs. So far, in Iran, due to the lack of recognition of genetic resources, a considerable breeding program has not been done on native plants. The study of the genetic diversity of violets as a native plant with ornamental and medicinal uses is the great importance in advancing the breeding goals of this plant. So, in the present study, nine populations of Viola spp . from different regions of Iran were used for evaluation of inter and intra-population genetic diversity with RAPD marker, and eight populations of them were used to evaluate morphological, vegetative, and reproductive characteristics. From ۱۱ used primers, ۱۴۵ bands which showed high resolution and their length was between ۲۵۰ to ۳۰۰۰ base pairs, were counted and used for RAPD analysis. According to the cluster analysis using the JACCARD similarity coefficient and UPGMA method, significant differences were found among populations. Molecular analysis of variance showed ۷۷ and ۲۳% inter and intra population genetic diversity, respectively. Principal component analysis classified effective characteristics in ۶ groups which justified ۸۹.۶۲% of total changes and in the cluster analysis of morphological traits, populations were classified into three groups in distance ۱۰. The results of our molecular and morphological analysis showed considerable diversity among violet populations in Iran, which can be used in future breeding programs.

Keywords:

DNA extraction , Classification , Cluster Analysis , Molecular variance , UPGMA