Comparison the Effect of Calcium Sulfate and Ferric Chloride in Controlling Liver Bleeding an Animal Model Study

Background and Objective: The control of parenchymal hemorrhage especially in liver parenchyma, despite improvements in surgical science, is still one of the challenges that surgeons are facing with. Therefore, introducing an effective method to control liver bleeding is of important research priority. This study aimed to compare the hemostatic effect of calcium sulfate and ferric chloride on controlling liver parenchymal tissue bleeding in rats. Materials and Methods: In this experimental study ۷۰ male Wistar rats were randomly allocated to seven groups. An incision, two centimeters (cm) long and half cm depth, was made on each rat’s liver and the hemostasis time was measured first using calcium sulfate and ferric chloride with different concentrations (۱۵%, ۲۵%, and ۵۰%) and then using the control method (i.e. control bleeding by simple suturing). Then, liver tissue was examined for pathological changes. Finally, hemostasis time was entered into SPSS software and analyzed using one-way ANOVA, Post hoc and Mann Whitney tests. Results: Hemostasis mean time in groups of calcium sulfate concentrations of ۵۰%, ۲۵% and ۱۵%, were ۸.۰۰±۰.۹۴, ۲۰.۷۰±۱.۸۸,and ۳۶.۱۰±۲.۲۷ seconds, respectively, and in groups of ferric chloride concentrations of ۵۰%, ۲۵% and ۱۵%, were ۱۵.۵۰±۱.۷۱, ۲۹.۴۰±۲.۰۶ and ۴۹.۲۰±۳.۳۲ seconds respectively. The hemostasis mean time in the control group (suture) was ۹۱/۳۰±۷.۳۰ seconds. The haemostatic time for different concentrations of calcium sulfate and ferric chloride were significantly less than the control group (P=۰.۰۰۴). There was a statistically significant difference between different concentrations of calcium sulfate and ferric chloride haemostatic time (P = ۰.۰۰۹). The pathologic examination showed the highest frequency of low grade inflammation based on the defined pathological grading. 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Keywords:

Keywords: Liver Hemorrhage , Hemostasis , Calcium Sulfate , Ferric Chloride , Rat