Evaluation of the Effects of Fullerene C۶۰ Nanoparticles on Oxidative Stress Parameters at Liver and Brain of Normal Rats

Background & Objective: The potent antioxidant property of fullerene C۶۰ nanoparticles and their derivatives has been demonstrated in a wide range of in vitro and in vivo studies. Hence, we examined the effects of fullerene C۶۰ on the oxidative stress parameters at brain and liver of rats in normal situation. Materials & Methods: The study was performed in two groups of Wistar rats (each group, n = ۶); normal and fullerene-treated normal animals. Treated rats received orally fullerene via oral gavage at dose of ۱ mg/kg/day for ۶۰ days. At termination of the study, the oxidative stress parameters were determined at brain and liver tissues, including the contents of glutathione (GSH) and malondialdehyde (MDA), and the activity of catalase (CAT) and superoxide dismutase (SOD). The t-test was used to analyze the data between two groups. Results: Fullerene C۶۰ treatment did not change blood glucose of treated rats compared to untreated rats. Fullerene C۶۰ significantly increased the value of CAT activity (by ۶۶%) and MDA levels (by ۶۸%), whereas decreased SOD activity (by ۳۳%) at liver of treated rats compared to untreated animals (P< ۰.۰۵). Fullerene administration significantly increased only CAT activity of brain in the treated rats (۰.۳۴±۰.۱۰ U/mg protein) compared to untreated animals (۰.۱۲±۰.۰۳ U/mg protein), (P<۰.۰۵). Conclusion: Our findings indicated that oral administration of fullerene C۶۰ nanoparticles differently changes the oxidative stress parameters in liver and brain at normal condition. It is suggested that these effects must be considered for application of these nanoparticles in various therapeutic purposes.

Keywords:

Antioxidant , Fullerene , Oxidative stress , Nanomaterials