Colorimetric method based on salt-induced aggregation of gold nanoparticles and aptamer does not work for detection of tacrolimus
Publish place: Nanomedicine Journal، Vol: 8، Issue: 3
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_NAMJ-8-3_008
تاریخ نمایه سازی: 19 تیر 1400
Abstract:
Objective(s): Tacrolimus (TAC) is used in autoimmune diseases, organ transplantation, and nephrotic syndrome treatment. Therapeutic drug monitoring (TDM) of TAC is critical since it has narrow therapeutic index. Thus, the development of an easy and sensitive method is critical for detecting TAC in blood samples.Materials and Methods: In this study, we aimed to design a fast, simple,and specific colorimetric sensor based on aptamer/AuNPs to detect. Initially, the aptamer was adsorbed on the surface of the gold nanoparticles (AuNPs). Results: Then, it was expected that when the aptamer bound to TAC, AuNPs were aggregated by salt, and the color changed from red to blue. However, in our study, color did not change, and NaCl could not aggregate the AuNPs. In this approach, the optical properties of AuNPs and high affinity of aptamer were used for the detection of TAC.Conclusion: However, according to our data, this colorimetric aptasensor was not appropriate for the detection of TAC.
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Authors
Atena Mansouri
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Noor Mohammad Danesh
Department of Passive Defense, Malek Ashtar University of Technology, Tehran, Iran
Mohammad Ramezani
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
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