Investigating the variety of cultivable lactobacilli in crops of the local chickens of Mazandaran province and investigating the capabilities of their hydrolytic and probiotic enzymes

Background and Aim : In poultry industries, two goals are considered: Increasing the growth rate of chickens by accelerating the digestive system by consuming microorganisms that produce hydrolytic enzymes and increasing the growth rate of chickens by preventing infectious diseases by using probiotics .The term "probiotic" refers to living microorganisms that enter the body of a living organism through the digestive tract and can survive and multiply there and have beneficial effects on the health of the host.In this study, the enzymatic activity and probiotic traits in lactobacilli that can be cultivated in the crops of local chickens in Mazandaran province have been investigated.Methods : In this study, at first, ۳۰ local chickens were collected, separating the crops, cultivating their samples on MRS Agar, purifying the gram-positive bacteria, The ability to produce hydrolytic were studied in them. These isolates were evaluated for primary probiotic tests.Results : In this study, ۲۲۰ bacteria were isolated from ۳۰ crop specimens of chickens, of which ۷۲ isolates (۳۳.۰۲%) were negative catalase and gram-positive rod, which were considered as lactic acid bacteriat. In the study of enzymatic activity,۱ isolate (۱.۳%) with protease activity, ۱ isolate (۱.۳%) with amylase activity, ۲ isolates (۲.۷%) with lipase activity were obtained. In the study of probiotic activity,۱۰ isolates (۱۳.۸۸%) without beta-hemolytic activity, ۴ isolates (۵.۵۵%) were able to tolerate pH=۲, ۷ isolates (۹.۷۲%) were able to tolerate pH=۴ and ۱ isolate (۱.۳۳%) was able to tolerate bile salts.Conclusion : Lactic acid bacteria are a physiological group and include optional gram-positive-anaerobic bacteria wich includes bacteria with probiotic ability. Most of lactic acid bacteria are weak in the activity of hydrolytic enzymes. In the study of Lutluf kabir et al., Only three species of Lactobacillus acidophilus and Lactobacillus vitilinus were isolated from chickens crops by amylase activity. The four isolates of lactic acid bacteria isolated in this study, which have been able to produce the amylase, protease and lipase.

Keywords:

amylase , esterase , lactic acid bacteria , probiotic , protease , crop , lipase , chicken , microflora