سیویلیکا را در شبکه های اجتماعی دنبال نمایید.

In silico Analysis and Modeling of ACP-MIP–PilQ Chimeric Antigen from Neisseria meningitidis Serogroup B

Publish Year: 1394
Type: Journal paper
Language: English
View: 295

This Paper With 10 Page And PDF Format Ready To Download

Export:

Link to this Paper:

Document National Code:

JR_RBMB-4-1_007

Index date: 1 September 2021

In silico Analysis and Modeling of ACP-MIP–PilQ Chimeric Antigen from Neisseria meningitidis Serogroup B abstract

Background: Neisseria meningitidis, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5–15% of adults. The aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type IV pilus biogenesis protein antigen (ACP-MIP-PilQ) from N. meningitidis serogroup B strain. Methods: Biochemical properties and multiple alignments were predicted by appropriate web servers. Secondary molecular structures were predicted based on Chou and Fasman, Garnier-Osguthorpe-Robson, and Neural Network methods. Tertiary modeling elucidated conformational properties of the chimeric protein. Proteasome cleavage and transporter associated with antigen processing (TAP) binding sites, and T- and B-cell antigenic epitopes, were predicted using bioinformatic web servers. Results: Based on our in silico and immunoinformatics analyses, the ACP-MIP-PilQ protein (AMP) can induce high-level cross-strain bactericidal activity. In addition, several immune proteasomal cleavage sites were detected. The 22 epitopes associated with MHC class I and class II (DR) alleles were confirmed in the AMP. Thirty linear B-cell epitopes as antigenic regions were predicted from the full-length protein. Conclusion: All predicted properties of the AMP indicate it could be a good candidate for further immunological in vitro and in vivo studies.

In silico Analysis and Modeling of ACP-MIP–PilQ Chimeric Antigen from Neisseria meningitidis Serogroup B Keywords:

In silico Analysis and Modeling of ACP-MIP–PilQ Chimeric Antigen from Neisseria meningitidis Serogroup B authors

Mehrdad Gholami

Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Alireza Salimi Chirani

Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mona Moshiri

Department of Pathobiology, Division of Microbiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Mansour Sedighi

Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Abazar Pournajaf

Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Masoud Tohidfar

Agricultural Biotechnology Research Institute of Iran, Tehran, Iran

Gholamreza Irajian

Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran