Evaluation of loop mediated isothermal amplification method (LAMP) for detection of Mycoplasma hominis in vaginal swab samples in comparison whit Real time PCR

Background and Aim : : Mycoplasma hominis as one of the etiologic agent of non-gonococcal urethritis in women, should be identified in order to early treatment that led to the patient does not suffer from serious complications such as pelvic inflammatory disease and infertility. With regard to the importance of M. hominis in genitourinary diseases, there’s need for development of reliable, simple and repeatable laboratory method in diagnosis. The aim of study was to establish a ۱۶srRNA-based LAMP method for identification of M. hominis from vaginal samples in comparison with Real Time PCR.Methods : The eighty vaginal samples were taken from patients and were evaluated by culture, Real-time PCR and LAMP diagnostic assays. Results : Totally, ۱۳ (۱۶.۳%), ۱۴ (۱۷.۵%) and ۲۵ (۳۱.۳%) samples were positive for M. hominis by culture, Real-time PCR and LAMP methods, respectively. The one of negative culture results diagnosed by real-time PCR, while the LAMP assay detected twelve positive among negative cultures. Also, the sensitivity, specificity, positive and negative predictive value for LAMP were calculated as ۱۰۰%, ۸۳.۳%, ۵۶% and ۱۰۰%,respectively.Conclusion : Based on our results, LAMP technique was more specific and sensitive than both Real time PCR and culture methods for diagnosis of M. hominis.

Keywords:

Mycoplasma hominis , LAMP , ۱۶srRNA , vaginal samples , Real-Time PCR.