An Evaluation of Antimicrobial Activity of Polysaccharides Extracts of Ganoderma lucidum on Growth Inhibition of Pseudomonas aeruginosa Isolated from Urinary Tract Infection

Background and Aim : Pseudomonas aeruginosa is an opportunistic pathogen and also one of the main causes of nosocomial infections. The purpose of this study was to evaluate the antimicrobial effect of polysaccharide extracts from Ganoderma lucidum on Pseudomonas aeruginosa bacterial infection which is resistant to common antibiotics.Methods : In this study, polysaccharide extracts of G. lucidum were initially extracted using seven different methods. Furthermore, antimicrobial tests such as the agar well diffusion test, Minimum Inhibitory Concentration (MIC), and Minimum Bacteriocidal Concentration (MBC) were conducted. In the next step, the drug resistance of microbial strains was determined, and the extract's ability to inhibit free radicals of (DPPH) was evaluated.Results : In this study, by analyzing the yield of extracted polysaccharides indicate that the Soxhlet extractor method with ۱۶% has the highest yield compared to other methods. According to the results of the diameter of the growth zone of inhibition for polysaccharide extract was found as ۱.۵ cm. Based on the findings, the MIC and MBC for polysaccharide extract were calculated to be ۰.۸۵۹ and ۶.۸۷۵ mg/ml, respectively. The results of urinary drug susceptibility testing indicated resistance to all tested antibiotics. The findings of the DPPH test showed that based on IC۵۰, its value for polysaccharide extract was obtained as ۶۴۷.۷۶ ?g/ml. Conclusion : The results of this study revealed that G. lucidum has a high antimicrobial ability and can also be suggested as a potential medical treatment for urinary tract infections.

Keywords:

Antimicrobial , Polysaccharides , G.lucidum , P. aeruginosa